Coding

Part:BBa_K1491027:Design

Designed by: Danielle Peters   Group: iGEM14_Carnegie_Mellon   (2014-09-19)


T7 RNA Polymerase with Dead ER Intein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2464
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2889
    Illegal AgeI site found at 2853
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2364


Design Notes

Control for T7 RNA Polyermase with ER intein (BBa_K1491025)


Source

T7 RNA Polymerase is BBa_K145001 and dead ER intein is BBa_K1491026

References